GK4, a G-protein-coupled receptor with a phosphatidylinositol phosphate kinase domain in Phytophthora infestans, is involved in sporangia development and virulence.
Identifieur interne : 001308 ( Main/Exploration ); précédent : 001307; suivant : 001309GK4, a G-protein-coupled receptor with a phosphatidylinositol phosphate kinase domain in Phytophthora infestans, is involved in sporangia development and virulence.
Auteurs : Chenlei Hua [Pays-Bas] ; Harold J G. Meijer ; Jeroen De Keijzer ; Wei Zhao ; Yuanchao Wang ; Francine GoversSource :
- Molecular microbiology [ 1365-2958 ] ; 2013.
Descripteurs français
- KwdFr :
- Feuilles de plante (microbiologie), Hyphae (croissance et développement), Maladies des plantes (microbiologie), Phosphates phosphatidylinositol (métabolisme), Phosphotransferases (génétique), Phosphotransferases (métabolisme), Phytophthora infestans (croissance et développement), Phytophthora infestans (enzymologie), Phytophthora infestans (métabolisme), Phytophthora infestans (pathogénicité), Protéines luminescentes (génétique), Protéines luminescentes (métabolisme), Récepteurs couplés aux protéines G (composition chimique), Récepteurs couplés aux protéines G (métabolisme), Régulation de l'expression des gènes au cours du développement (MeSH), Solanum tuberosum (microbiologie), Sporanges (croissance et développement), Spores (croissance et développement), Tabac (microbiologie).
- MESH :
- composition chimique : Récepteurs couplés aux protéines G.
- croissance et développement : Hyphae, Phytophthora infestans, Sporanges, Spores.
- enzymologie : Phytophthora infestans.
- génétique : Phosphotransferases, Protéines luminescentes.
- microbiologie : Feuilles de plante, Maladies des plantes, Solanum tuberosum, Tabac.
- métabolisme : Phosphates phosphatidylinositol, Phosphotransferases, Phytophthora infestans, Protéines luminescentes, Récepteurs couplés aux protéines G.
- pathogénicité : Phytophthora infestans.
- Régulation de l'expression des gènes au cours du développement.
English descriptors
- KwdEn :
- Gene Expression Regulation, Developmental (MeSH), Hyphae (growth & development), Luminescent Proteins (genetics), Luminescent Proteins (metabolism), Phosphatidylinositol Phosphates (metabolism), Phosphotransferases (genetics), Phosphotransferases (metabolism), Phytophthora infestans (enzymology), Phytophthora infestans (growth & development), Phytophthora infestans (metabolism), Phytophthora infestans (pathogenicity), Plant Diseases (microbiology), Plant Leaves (microbiology), Receptors, G-Protein-Coupled (chemistry), Receptors, G-Protein-Coupled (metabolism), Solanum tuberosum (microbiology), Sporangia (growth & development), Spores (growth & development), Tobacco (microbiology).
- MESH :
- chemical , chemistry : Receptors, G-Protein-Coupled.
- chemical , genetics : Luminescent Proteins, Phosphotransferases.
- enzymology : Phytophthora infestans.
- growth & development : Hyphae, Phytophthora infestans, Sporangia, Spores.
- chemical , metabolism : Luminescent Proteins, Phosphatidylinositol Phosphates, Phosphotransferases, Phytophthora infestans, Receptors, G-Protein-Coupled.
- microbiology : Plant Diseases, Plant Leaves, Solanum tuberosum, Tobacco.
- pathogenicity : Phytophthora infestans.
- Gene Expression Regulation, Developmental.
Abstract
For dispersal and host infection plant pathogens largely depend on asexual spores. Pathogenesis and sporulation are complex processes that are governed by cellular signalling networks including G-protein and phospholipid signalling. Oomycetes possess a family of novel proteins called GPCR-PIPKs (GKs) that are composed of a seven-transmembrane spanning (7-TM) domain fused to a phosphatidylinositol phosphate kinase (PIPK) domain. Based on this domain structure GKs are anticipated to link G-protein and phospholipid signal pathways; however, their functions are currently unknown. Expression analyses of the 12 GK genes in Phytophthora infestans and their orthologues in Phytophthora sojae, revealed differential expression during asexual development. PiGK1 and PiGK4 were fused to monomeric red fluorescent protein (mRFP) and ectopically expressed in P. infestans. In growing hyphae different subcellular distribution patterns were observed indicating that these two GKs act independently during development. We focused on the functional analyses of PiGK4. Its localization suggested involvement in cell differentiation and elongation and its 7-TM domain showed a canonical GPCR membrane topology. Silencing of GK4 and overexpression of full-length and truncated constructs in P. infestans revealed that PiGK4 is not only involved in spore germination and hyphal elongation but also in sporangia cleavage and infection.
DOI: 10.1111/mmi.12190
PubMed: 23448716
Affiliations:
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phosphatidylinositol (métabolisme)</term><term>Phosphotransferases (génétique)</term><term>Phosphotransferases (métabolisme)</term><term>Phytophthora infestans (croissance et développement)</term><term>Phytophthora infestans (enzymologie)</term><term>Phytophthora infestans (métabolisme)</term><term>Phytophthora infestans (pathogénicité)</term><term>Protéines luminescentes (génétique)</term><term>Protéines luminescentes (métabolisme)</term><term>Récepteurs couplés aux protéines G (composition chimique)</term><term>Récepteurs couplés aux protéines G (métabolisme)</term><term>Régulation de l'expression des gènes au cours du développement (MeSH)</term><term>Solanum tuberosum (microbiologie)</term><term>Sporanges (croissance et développement)</term><term>Spores (croissance et développement)</term><term>Tabac (microbiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Receptors, G-Protein-Coupled</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Luminescent Proteins</term><term>Phosphotransferases</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Récepteurs couplés aux protéines G</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Hyphae</term><term>Phytophthora infestans</term><term>Sporanges</term><term>Spores</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Hyphae</term><term>Phytophthora infestans</term><term>Sporangia</term><term>Spores</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Phosphotransferases</term><term>Protéines luminescentes</term></keywords><keywords scheme="MESH" 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scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Developmental</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Régulation de l'expression des gènes au cours du développement</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">For dispersal and host infection plant pathogens largely depend on asexual spores. Pathogenesis and sporulation are complex processes that are governed by cellular signalling networks including G-protein and phospholipid signalling. Oomycetes possess a family of novel proteins called GPCR-PIPKs (GKs) that are composed of a seven-transmembrane spanning (7-TM) domain fused to a phosphatidylinositol phosphate kinase (PIPK) domain. Based on this domain structure GKs are anticipated to link G-protein and phospholipid signal pathways; however, their functions are currently unknown. Expression analyses of the 12 GK genes in Phytophthora infestans and their orthologues in Phytophthora sojae, revealed differential expression during asexual development. PiGK1 and PiGK4 were fused to monomeric red fluorescent protein (mRFP) and ectopically expressed in P. infestans. In growing hyphae different subcellular distribution patterns were observed indicating that these two GKs act independently during development. We focused on the functional analyses of PiGK4. Its localization suggested involvement in cell differentiation and elongation and its 7-TM domain showed a canonical GPCR membrane topology. Silencing of GK4 and overexpression of full-length and truncated constructs in P. infestans revealed that PiGK4 is not only involved in spore germination and hyphal elongation but also in sporangia cleavage and infection.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23448716</PMID><DateCompleted><Year>2013</Year><Month>09</Month><Day>25</Day></DateCompleted><DateRevised><Year>2013</Year><Month>04</Month><Day>11</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1365-2958</ISSN><JournalIssue CitedMedium="Internet"><Volume>88</Volume><Issue>2</Issue><PubDate><Year>2013</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Molecular microbiology</Title><ISOAbbreviation>Mol Microbiol</ISOAbbreviation></Journal><ArticleTitle>GK4, a G-protein-coupled receptor with a phosphatidylinositol phosphate kinase domain in Phytophthora infestans, is involved in sporangia development and virulence.</ArticleTitle><Pagination><MedlinePgn>352-70</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/mmi.12190</ELocationID><Abstract><AbstractText>For dispersal and host infection plant pathogens largely depend on asexual spores. Pathogenesis and sporulation are complex processes that are governed by cellular signalling networks including G-protein and phospholipid signalling. Oomycetes possess a family of novel proteins called GPCR-PIPKs (GKs) that are composed of a seven-transmembrane spanning (7-TM) domain fused to a phosphatidylinositol phosphate kinase (PIPK) domain. Based on this domain structure GKs are anticipated to link G-protein and phospholipid signal pathways; however, their functions are currently unknown. Expression analyses of the 12 GK genes in Phytophthora infestans and their orthologues in Phytophthora sojae, revealed differential expression during asexual development. PiGK1 and PiGK4 were fused to monomeric red fluorescent protein (mRFP) and ectopically expressed in P. infestans. In growing hyphae different subcellular distribution patterns were observed indicating that these two GKs act independently during development. We focused on the functional analyses of PiGK4. Its localization suggested involvement in cell differentiation and elongation and its 7-TM domain showed a canonical GPCR membrane topology. Silencing of GK4 and overexpression of full-length and truncated constructs in P. infestans revealed that PiGK4 is not only involved in spore germination and hyphal elongation but also in sporangia cleavage and infection.</AbstractText><CopyrightInformation>© 2013 Blackwell Publishing Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hua</LastName><ForeName>Chenlei</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Laboratory of Phytopathology, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Meijer</LastName><ForeName>Harold J G</ForeName><Initials>HJ</Initials></Author><Author ValidYN="Y"><LastName>de Keijzer</LastName><ForeName>Jeroen</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Wei</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Yuanchao</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Govers</LastName><ForeName>Francine</ForeName><Initials>F</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>03</Month><Day>11</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Mol Microbiol</MedlineTA><NlmUniqueID>8712028</NlmUniqueID><ISSNLinking>0950-382X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008164">Luminescent Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018129">Phosphatidylinositol Phosphates</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D043562">Receptors, G-Protein-Coupled</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C413662">red fluorescent protein</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.-</RegistryNumber><NameOfSubstance UI="D010770">Phosphotransferases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D018507" MajorTopicYN="N">Gene Expression Regulation, Developmental</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D025301" MajorTopicYN="N">Hyphae</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008164" MajorTopicYN="N">Luminescent Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018129" MajorTopicYN="N">Phosphatidylinositol Phosphates</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010770" MajorTopicYN="N">Phosphotransferases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D055750" MajorTopicYN="N">Phytophthora infestans</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000472" MajorTopicYN="Y">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D043562" MajorTopicYN="N">Receptors, G-Protein-Coupled</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011198" MajorTopicYN="N">Solanum tuberosum</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D058432" MajorTopicYN="N">Sporangia</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013170" MajorTopicYN="N">Spores</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="accepted"><Year>2013</Year><Month>02</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>3</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>3</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>9</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23448716</ArticleId><ArticleId IdType="doi">10.1111/mmi.12190</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Pays-Bas</li></country><region><li>Gueldre (province)</li></region><settlement><li>Wageningue</li></settlement><orgName><li>Université de Wageningue</li></orgName></list><tree><noCountry><name sortKey="De Keijzer, Jeroen" sort="De Keijzer, Jeroen" uniqKey="De Keijzer J" first="Jeroen" last="De Keijzer">Jeroen De Keijzer</name><name sortKey="Govers, Francine" sort="Govers, Francine" uniqKey="Govers F" first="Francine" last="Govers">Francine Govers</name><name sortKey="Meijer, Harold J G" sort="Meijer, Harold J G" uniqKey="Meijer H" first="Harold J G" last="Meijer">Harold J G. Meijer</name><name sortKey="Wang, Yuanchao" sort="Wang, Yuanchao" uniqKey="Wang Y" first="Yuanchao" last="Wang">Yuanchao Wang</name><name sortKey="Zhao, Wei" sort="Zhao, Wei" uniqKey="Zhao W" first="Wei" last="Zhao">Wei Zhao</name></noCountry><country name="Pays-Bas"><region name="Gueldre (province)"><name sortKey="Hua, Chenlei" sort="Hua, Chenlei" uniqKey="Hua C" first="Chenlei" last="Hua">Chenlei Hua</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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